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Title   한국인 간암조직 및 간암세포주에서 p53 유전자의 이상에 관한 연구 ( p53 Gene Mutation in Hepation in Hepatocellular Carcinoma from Korean Patients and in Established Hepatocellular Carcinoma Cell Lines )
Publicationinfo   1993 Jan; 025(03): 359-368.
Key_word   Hepatocellular carcinoma, p53 gene, Mutation
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Abstract   Inactivation of tumor suppressor gene is believed to be important in the development af many hyman malignancies. Recently, several lines of evidence have indicated that the wild- type p53 gene, located at chromosome 17pl3.l, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating noramal p53 function in a dominant negative fashion. In addition to gross DNA abnormalities, many different point mutations in highly conserved regions of the open reading frame have been observed in a vari- ety of common cancers, such as lung, colon, and breast cancers. In hepatocellular carcinoma(HCC) from Quidong in China and southern Africa, known mutations of the p53 gene ihclude selective guaine to thymine(G to T) transversions, which tend to cluster at codon 249. This mutational specificity could reflect exposure to specific carcinogen such as aflatoxin Bl. In the current study, Southern blot analysis, PCR-SSCP, and nucleotide sequencing were performed to evaluate the loss of heterozygosity(LOH) on chromosome 17p and structural abnormalities of p53 gene in the hepatoma tissues from Korean patients and in established HCC cell lines. A cell line sample of Hep-3B showed partial deletion of p53 gene but contained no mutation in the open reading frame. Three HCC tissue samples out of seven informative cases demon- strated LOH at chromosome 17p but no p53 gene mutation in those samples. In this study, only one out of thirteen HCC tissue sarnples from Korean patients revelaled p53 gene mutation at codon 244, which was G to A transition(from GGC to GAC). p53 gene mutation is relatively rare in hepatoma from Korean patients especially at codon 249. The mutational pattern and frequency of p53 gene in the hepatoma suggested that hepatitis B viral infection appears to be more important than aflatoxin Bl in hepato-carcinogenesis in Korea.
저 자   김주항(Joo Hang Kim),박주배(Joo Bae Park),Tetsuya Mitsudomi,최정주(Jung Joo Choi),유내춘(Nae Chun Yoo),최진혁(Jin Hyuk Choi),임호영(Ho Yeong Lim),노재경(Jae Kyung Roh),이경식(Kyong Sik Lee),김병수(Byung Soo Kim)